کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8553712 | 1562692 | 2018 | 47 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Property characterization of reconstructed human epidermis equivalents, and performance as a skin irritation model
ترجمه فارسی عنوان
مشخصه اموال معادل اپی ترومبوس بازسازی شده انسان و عملکرد آن به عنوان یک مدل تحریک پوست
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کلمات کلیدی
GHSET50within laboratory reproducibilityNHKVRMOECD TGPBSECVAMSDSWLRRHEBSA - BSAbovine serum albumin - آلبومین سرم گاوPerformance standard - استاندارد عملکردReconstructed human epidermis - اپی ادیس انسانی بازسازی شدهUpper limit - حد بالاlower limit - حد پایینhematoxylin and eosin staining - رنگ آمیزی هماتوکسیلین و ائوزینsodium dodecyl-sulfate - سدیم دودسیل سولفاتthree dimensional - سه بعدیGlobally Harmonized System - سیستم هماهنگ جهانیphosphate buffer saline - فسفات بافر شورEuropean Centre for the Validation of Alternative Methods - مرکز اروپا برای اعتبار سنجی روش های جایگزینHuman skin - پوست انسانKeratinocytes - کراتینوسیتnormal human keratinocytes - کراتینوسیت های طبیعی طبیعی انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
In recent years, in vitro skin models combining cell biology and tissue engineering have been developed in order to replace animal models for toxicological studies and to serve as research support to better understand skin biology. This study reports the development and characterization of a epidermal tissue equivalent meant to be used to develop and to evaluate the effect of applied cosmetic ingredients, and for alternative toxicological testing. This epidermis equivalent model was characterized relative to the morphological characteristics of short- and long-term maintained tissues by performing histological studies. We also studied the integrity of the epidermal barrier. Finally, with the goal of validating its use as a skin irritation test, we studied the irritation potential of 20 chemical references listed in OECD Test Guideline N°439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method). In 2015, OECD officially published the updated version of the Validated Reference Method (VRM) that uses reconstructed human epidermis models for irritation testing, thus offering the possibility for proposed putative similar test methods to obtain a validation agreement through Performance Standards-based validation. In this study, we observed that the epidermal equivalent we developed showed similarities to human in vivo skin, based on the analyzed parameters. Moreover, its performances as a skin irritation test were similar to the ones described in the OECD Test Guideline N°439.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 53, December 2018, Pages 45-56
Journal: Toxicology in Vitro - Volume 53, December 2018, Pages 45-56
نویسندگان
Christophe Capallere, Christelle Plaza, Céline Meyrignac, Marianne Arcioni, Marie Brulas, Valère Busuttil, Imane Garcia, Ãric Bauza, Jean-Marie Botto,