کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8553924 | 1562697 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acute effects of lead on porcine neonatal Sertoli cells in vitro
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کلمات کلیدی
ASMAFSH-R3β-Hydroxysteroid dehydrogenaseJnkITSNaClOSHARIPA3β-HSDSCsECLc-Jun N-terminal kinase - C-Jun N-terminal kinaseERK1/2 - ERK1 / 2MAPK - MAPKMTT - MTTanti-smooth muscle antibody - آنتی بادی عضلانی ضد صافAkt - آکتOccupational Safety and Health Administration - اداره ایمنی و بهداشت حرفه ایLead acetate - استات سربinsulin-transferrin-selenium - انسولین انتقال دهنده سلنیومAMH - اوradioimmunoprecipitation assay buffer - بافر تست radioimmunoprecipitationenhanced chemiluminescence - بهبود شیمیایی لومنSodium chloride - سدیم کلریدSertoli cells - سلولهای SertoliToxicity - سمی بودنAnti-Müllerian hormone - هورمون Anti-Mellerianfollicle stimulating hormone - هورمون تحریک کننده فولیکولFSH - هورمون محرکه فولیکولی Western blotting - وسترن بلاتینگprotein kinase B - پروتئین کیناز Bmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استextracellular signal-regulated kinase 1/2 - کیناز 1/2 تنظیم سیگنال خارج سلولیFollicle stimulating hormone receptor - گیرنده هورمون تحریک کننده فولیکول
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Environmental pollution is one of the main factors responsible for reducing fertility in males. Lead is one of the major heavy metal contaminants that impairs several organs; it preferentially accumulates in male reproductive organs and alters sperm quality both in vivo and in vitro. However, the underlying mechanisms remain unclear. Sertoli cells (SCs) provide structural and physiological support to spermatogenic cells within seminiferous tubules. Therefore, changes in SCs affect the developing germ cells and alter spermatogenesis. This study aimed to assess whether exposure to subtoxic doses of adversely affects SC functioning in higher mammals. Purified and functional porcine neonatal SCs were exposed to lead acetate at three different concentrations. Lead exposure decreased the mRNA expression and protein levels of inhibin B and anti-Mullerian hormone (AMH) compared to control, indicating loss of FSH-r integrity in terms of 17-β-oestradiol production under FSH stimulation. In addition, we observed an increase in the mRNA levels of Akt and mTOR, and the phosphorylation of p38 and Akt in SCs exposed to lead at all concentrations compared to unexposed control SCs. In conclusion, lead is toxic to SCs, even at low concentrations, and is expected to alter spermatogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 48, April 2018, Pages 45-52
Journal: Toxicology in Vitro - Volume 48, April 2018, Pages 45-52
نویسندگان
Francesca Mancuso, Iva Arato, Cinzia Lilli, Catia Bellucci, Maria Bodo, Mario Calvitti, Maria Chiara Aglietti, Marco dell'Omo, Claudio Nastruzzi, Riccardo Calafiore, Giovanni Luca, Lorella Marinucci,