کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8553947 | 1562695 | 2018 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heterocyclic organobismuth (III) compounds containing an eight-membered ring: Inhibitory effects on cell cycle progression
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کلمات کلیدی
DMEMMTOCDAPITBSEGTAPVDFPBS4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده Dulbeccoethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid - اتیلن گلیکول بیس (β-آمینویل اتر) -N، N، N '، N'-tetraacetic اسیدsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSpindle checkpoint - ایست بازرسی اسپیندلTris-buffered saline - تریس بافر شورMitotic arrest - دستگیری متیوتیکPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریmicrotubule-organizing center - مرکز میکروتوبولی سازماندهیPolyvinylidene fluoride - پلی وینیلیدین فلوراید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We previously showed that heterocyclic organobismuth compounds have excellent antimicrobial and antitumor potential. These compounds structurally consist of either six- or eight-membered rings. Previous research has shown that bi-chlorodibenzo[c,f][1,5]thiabismocine (Compound 3), an eight-membered ring, induced G2/M arrest via inhibition of tubulin polymerization in HeLa cells. Additionally, N-tert-butyl-bi-chlorodi-benzo[c,f][1,5]azabismocine (Compound 1), another eight-membered ring, exhibited higher cytotoxicity than Compound 3 against several cancer cell lines, including HeLa and K562. Finally, bi-chlorophenothiabismin-S,S-dioxide (Compound 5), a six-membered ring, exhibited lower antitumor activity than eight-membered ring compounds. In this study, we investigated the antimitotic activity of Compounds 1 and 5 in HeLa cells. At low concentrations, (0.1 and 0.25â¯Î¼M), Compound 1 inhibited cell growth and arrested the cell cycle in mitosis. However, 0.5â¯Î¼M Compound 1 exhibited no antimitotic activity. Conversely, Compound 5 weakly inhibited cell growth and did not markedly arrest the cell cycle. Flow cytometry showed that Compound 1 arrested the cell cycle at G2/M, resulting in apoptosis. Compound 1 inhibited tubulin polymerization as revealed by a cell-free assay, and both Compounds 1 and 3 inhibited microtubule spindle formation and chromosome alignment during prometaphase. These results suggest that eight-membered ring-containing organobismuth compounds can induce mitotic arrest by perturbing spindle dynamics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 50, August 2018, Pages 172-178
Journal: Toxicology in Vitro - Volume 50, August 2018, Pages 172-178
نویسندگان
Katsuya Iuchi, Tatsuo Yagura,