کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8631149 | 1568848 | 2018 | 40 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miRNAome, mRNAome and degradome analysis of Tibetan minipigs anterior pituitary
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کلمات کلیدی
POMCmessage RNAmRNA-SeqGnRHRpro-opiomelanocortinLHbGH1RPKMPRLreads per kilobase of transcript per million mapped readsPOU1F1qRT-PCRCGAfshbmRNA sequencingGHRHRmRNAHMGA13′-UTR - 3'-UTR3′-untranslated region - 3'-ناحیه ترجمه نشدهcDNA - cDNAComplementary DNA - DNA تکمیلیmicro RNA - RNA میکروTargets - اهدافPare - به نظر می رسدPig - خوکKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوRIN - رینRNA Integrity Number - شماره یکپارچه RNAMiRNA - میکروRNA، ریزآرانای، miRNAGene ontology - هستیشناسی ژنیGrowth hormone - هورمون رشدGrowth hormone releasing hormone receptor - هورمون رشد هورمون آزاد کننده گیرنده هورمونAnterior pituitary - هیپوفیز قدامیquantitative reverse transcription polymerase chain reaction - واکنش زنجیره ای پلی مراز رونویسی معکوسProlactin - پرولاکتین gonadotropin releasing hormone receptor - گیرنده هورمون آزاد کننده گونادوتروپین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Tibetan minipig is an important animal model for human diseases. The anterior pituitary is the master gland responsible for growth, reproduction, and metabolism and is regulated by thousands of miRNAs/mRNAs molecules. However, little is known about miRNAs and their relationships with mRNAs in Tibetan minipig anterior pituitary. Using microarray and mRNA-Sequencing, we identified 203 miRNAs and 12,040 mRNA transcripts from the anterior pituitary of Tibetan minipigs. These miRNAs were corresponding to 194 hairpin precursors, 25 miRNA clusters and 24 miRNA families. In addition, 64 intragenic miRNAs were annotated. Using three bioinformatic algorithms (TargetScan, miRanda and RNAhybrid), 359,184 possible miRNA-mRNA interactions were predicted, and an integrated network of miRNAs and pituitary-specific mRNA transcripts was established. To validate the predicted results, the degradome sequencing was employed to confirm miRNA-mRNA interactions, totally, 30 miRNA-mRNA pairs were identified. The present study provided a general overview of miRNA and mRNA annotation in Tibetan minipig anterior pituitary and established a miRNA-mRNA interactions database at the whole genome scale, which helps shed light on the molecular mechanisms in the anterior pituitary of pigs even other mammals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 259, 1 April 2018, Pages 104-114
Journal: General and Comparative Endocrinology - Volume 259, 1 April 2018, Pages 104-114
نویسندگان
Rui-Song Ye, Meng Li, Ting Chen, Xiao-Chen Wei, Qi-En Qi, Xiao Cheng, Chao-Yun Li, Qing-Yan Jiang, Qian-Yun Xi, Yong-Liang Zhang,