کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8633965 | 1569088 | 2018 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
l-Arginine supplementation prevents intestinal epithelial barrier breakdown under heat stress conditions by promoting nitric oxide synthesis
ترجمه فارسی عنوان
مکمل ال-آرژینین مانع از شکستن موانع اپیتلیال روده در شرایط تنش گرما شده از طریق ترویج سنتز اکسید نیتریک
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کلمات کلیدی
hsp70PBSDMEMiNOSTEERHO-1Caco-2qRT-PCRHeme oxygenase-1NOS3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideE-cadherin - E-Cadherinl-NAME - L-NAMEl-NG-Nitroarginine methyl ester - L-NG-Nitroarginine متیل استرMTT - MTTl-arginine - آرژینین الtight junctions - اتصالات محکمHeat stress - استرس گرماییl-Arg - ال-آرژینینanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییlucifer yellow - لوسیفر زردPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریtransepithelial electrical resistance - مقاومت الکتریکی transepithelialNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استheat shock protein 70 - پروتئین شوک حرارت 70adherens junctions - پیوندها را پیگیری می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
چکیده انگلیسی
Heat stress (HS) induced by exposure to high ambient temperatures or prolonged excessive physical activities is known to primarily induce deleterious effects on the intestinal integrity by disrupting junctional complexes. Considering the association of l-arginine (l-Arg) with the improvement of gut function, the hypothesis of this study was to assess whether l-Arg supplementation can prevent the intestinal barrier disruption under HS conditions and to understand whether the l-Arg-induced effects are associated with maintaining nitric oxide (NO) as the major product of l-Arg metabolism. For this study, human colorectal adenocarcinoma (Caco-2) cells grown on Transwell inserts were pretreated with different l-Arg concentrations (0.4, 1, and 4 mmol/L), and after exposure to HS, markers of intestinal barrier integrity, stress-related markers, and NO levels were determined. l-Arg deprivation markedly increased the mRNA expression of heat shock protein 70 and heme-oxygenase-1 under HS conditions. The HS-induced drop in transepithelial electrical resistance values and increase in Lucifer Yellow permeability could be prevented by 4 mmol/L l-Arg supplementation. In turn, l-Arg mitigated the downregulation and delocalization of adherens junction protein E-cadherin in HS-exposed cells. NO and inducible NO synthase levels were significantly decreased in HS-exposed cells, whereas pretreatment with 4 mmol/L l-Arg prevented this decrease. Inhibition of inducible NO synthase by the NO synthase inhibitor l-NG-nitroarginine methyl ester abrogated the effect of l-Arg on preserving intestinal integrity under HS conditions as measured by transepithelial electrical resistance, Lucifer Yellow flux, and E-cadherin expression. In summary, l-Arg supplementation protects the intestinal epithelial integrity, at least partly, by maintaining NO synthesis under HS conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition Research - Volume 57, September 2018, Pages 45-55
Journal: Nutrition Research - Volume 57, September 2018, Pages 45-55
نویسندگان
Soheil Varasteh, Saskia Braber, Aletta D. Kraneveld, Johan Garssen, Johanna Fink-Gremmels,