کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8634900 | 1569136 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High resolution melting (HRM)-based STMS marker analysis for rapid identification of radiation induced mutants of sugarcane
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کلمات کلیدی
PAGESTMSTRAPEMSRFLPAFLPRAPDHRMCTABEthyl methane sulfonate - اتیل متان سولفوناتpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدcetyl trimethyl ammonium bromide - بتدریستیل آمونیوم برومیدMutation detection - تشخیص جهشRandom amplification of polymorphic DNA - تقویت تصادفی DNA پلیمورفیکHigh-resolution melting - ذوب با وضوح بالاHigh resolution melting - ذوب شدن با وضوح بالاMelting curve - منحنی ذوبpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازAmplified fragment length polymorphism - پلی مورفیسم طول قطعه تقویت شده
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High resolution melting (HRM)-based STMS marker analysis for rapid identification of radiation induced mutants of sugarcane High resolution melting (HRM)-based STMS marker analysis for rapid identification of radiation induced mutants of sugarcane](/preview/png/8634900.png)
چکیده انگلیسی
Molecular marker based selection has become the method of choice to find undiscoverable natural variations and induced mutations in plants. Routine gel based molecular marker screening procedures involve complex steps which require considerable investment of time, money and efforts. Most often, genomic configuration of a plant species complicates the ultimate output of such screening programme. Therefore, a reliable and rapid high-throughput method is required to facilitate ease in identification of variants in a large number of samples. Here we demonstrate the application of a high-resolution melting (HRM) method to detect gamma radiation induced mutants of sugarcane (Saccharum spp.) which is a complex aneu-polyploidy plant. We have screened nine distinct mutants using sequence-tagged microsatellite site (STMS) markers and the analyses based on gel electrophoresis and HRM curve were compared. All the six STMS microsatellite markers coupled with HRM analysis were found highly informative and generated a unique melting curve for each of the marker tested. Of these, two markers NKSCSSR 22 and NKSCSSR 42 exhibited differential melting curve among the mutants and these results were well correlated with the gel based banding pattern. Taken together these results highlighted potential of HRM based marker screening and demonstrated reliability and robustness in detecting induced mutations in a complex polyploidy plant like sugarcane.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agri Gene - Volume 8, June 2018, Pages 37-44
Journal: Agri Gene - Volume 8, June 2018, Pages 37-44
نویسندگان
Mirajkar Shriram J., Vaidya Eknath R., Suprasanna Penna,