کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8646207 | 1570073 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DNA methyltransferases and tRNA methyltransferase DNMT2 in developing pig brain - expression and promoter methylation
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کلمات کلیدی
NCBIGAPDHRT-PCRDnmtDNA methyl transferaseReverse transcriptase PCR - PCR معکوس رونویسیPig - خوکMethylation - متیلاسیونNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیBrain - مغزpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: DNA methyltransferases and tRNA methyltransferase DNMT2 in developing pig brain - expression and promoter methylation DNA methyltransferases and tRNA methyltransferase DNMT2 in developing pig brain - expression and promoter methylation](/preview/png/8646207.png)
چکیده انگلیسی
DNA methylation constitutes an epigenetic mechanism used by cells to control gene expression and chromatin organization. DNA methylation is a biochemical process, catalyzed by DNA methyltransferases, where a methyl group is added to a nucleotide base. In mammals, DNA methylation is essential for normal development. Here we report on the developmental expression of DNA methyltransferases and DNMT2, a tRNA methyltransferase, in various brain tissues in porcine embryos. Expressions of DNMT1, DNMT2, DMNT3A and DNMT3B transcripts were determined by qRT-PCR in five different brain tissues at four or five time points in embryo development. Low transcript levels from all these genes were detected as early as 60â¯days of gestation in all tissues examined. From 60 to 115â¯days of gestation, a reduction in DNMT1 and DMNT3A and DNMT3B mRNA was seen in brain stem, hippocampus, basal ganglia, cortex and cerebellum. No significant changes were seen for DNMT2 mRNA. In heart, a bimodal expression was observed for all four DNMTs analyzed. Analysis of DNA methylation status of two selected regions in the DNMT3A promoter revealed a very low degree of methylation (<3%). It was concluded that DNA methylation of the examined region of the DNMT3A gene is not determining expression of its transcript.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Reports - Volume 11, June 2018, Pages 42-51
Journal: Gene Reports - Volume 11, June 2018, Pages 42-51
نویسندگان
Knud Larsen, Kaja Kjær Kristensen, Henrik Callesen,