کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8646759 | 1570141 | 2018 | 49 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bioinformatics analysis of ROP8 protein to improve vaccine design against Toxoplasma gondii
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کلمات کلیدی
GORMICIEDBPDBIC50NCBIIFN-γIgEROPCTLThree-dimensionalANNSAGIgGPTMACCgrand average of hydropathicitySurface antigen - آنتی ژن سطحیpost-translational modification - اصلاح post-translationalImmunoglobulin E - ایمونوگلوبولین Eimmunoglobulin G - ایمونوگلوبولین Ginterferon-γ - اینترفرون-γGRA - بازیT. gondii - ت.گندیدیBioinformatics analysis - تجزیه و تحلیل بیوانفورماتیکToxoplasma gondii - توکسوپلاسما گوندیcluster of differentiation - خوشه تمایزArtificial Neural Network - شبکه عصبی مصنوعیconfidence interval - فاصله اطمینانcytotoxic T lymphocyte - لنفوسیت T سیتوتوکسیکSVM - ماشین بردار پشتیبانیSupport vector machine - ماشین بردار پشتیبانیMacrophages - ماکروفاژها،درشت خوارهاMHC - مجموعه سازگاری بافتی اصلیmajor histocompatibility complex - مجموعه سازگاری بافتی اصلیNational Centre for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیIsoelectric point - نقطه ایزوالکتریکhalf maximal inhibitory concentration - نیمه حداکثر غلظت مهاریVaccine - واکسنparasitophorous vacuole - واکسن پارازیتوفورMolecular weight - وزن مولکولیImmune Epitope Database - پایگاه اطلاعات ایمنیProtein Data Bank - پروتئین بانک اطلاعاتیGRAVY - گرگ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Rhoptry proteins (ROPs) are involved in the different stages of Toxoplasma gondii (T. gondii) invasion and are also critical for survival within host cells. ROP8 is expressed in the early stages of infection and have a key role in the parasitophorous vacuole (PV) formation. In this paper, we have combined several bioinformatics online servers for immunogenicity prediction of ROP8 protein. In this study, several bioinformatics approaches were used to analyze the different aspects of ROP8 protein, including the physico-chemical properties, transmembrane domain, subcellular localization, secondary and tertiary structure, B and T-cell potential epitopes, and other important characteristics of this protein. The findings showed that ROP8 protein had 60 potential post-translational modification sites. Also, only one transmembrane domain was recognized for this protein. The secondary structure of ROP8 protein comprises 33.04% alpha-helix, 18.26% extended strand, and 48.70% random coil. Moreover, several potential B and T-cell epitopes were identified for ROP8. In addition, the obtained findings from antigenicity and allergenicity evaluation remarked that this protein is immunogenic and non-allergen. Based on the results of Ramachandran plot, 94.8%, 4.1%, and 1.1% of amino acid residues were incorporated in the favored, allowed, and outlier regions, respectively. This paper provides a foundation for further investigations, and laid a theoretical basis for the development of an appropriate vaccine against toxoplasmosis. More studies are needed experimentally using the ROP8 alone or in combination with other antigens in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 62, August 2018, Pages 193-204
Journal: Infection, Genetics and Evolution - Volume 62, August 2018, Pages 193-204
نویسندگان
Masoud Foroutan, Fatemeh Ghaffarifar, Zohreh Sharifi, Abdolhosein Dalimi, Majid Pirestani,