کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8647740 | 1570309 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isoform-specific subcellular localization of Zea mays lipoxygenases and oxo-phytodienoate reductase 2
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Plant 9- and 13-lipoxygenases (9- and 13-LOXs) function in an early step of the oxylipin biosynthesis pathway. Here we report diverse subcellular localization profiles of four maize LOX family proteins and one oxo-phytodienoate reductase 2, subcellular localization of which could not be predicted by bioinformatics tools. Fluorescent protein-tagged LOX proteins were transiently expressed in maize B73 protoplasts as well as in stably transformed Arabidopsis thaliana. Localization profiles of maize 9-LOXs were more diverse than that of reported 13-LOX isoforms and spanned cytoplasm, plastids and tonoplasts, suggesting compartmentation of different oxylipin production inside of the cells. Localization profiles of maize 9-LOX were mostly consistent between maize and Arabidopsis, suggesting subcellular targeting mechanisms of these isoforms are conserved between monocots and dicots.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Gene - Volume 13, March 2018, Pages 36-41
Journal: Plant Gene - Volume 13, March 2018, Pages 36-41
نویسندگان
Jordan P. Tolley, Yukihiro Nagashima, Zachary Gorman, Michael V. Kolomiets, Hisashi Koiwa,