کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
870006 909846 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A disposable electrochemical immunosensor for flow injection immunoassay of carcinoembryonic antigen
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A disposable electrochemical immunosensor for flow injection immunoassay of carcinoembryonic antigen
چکیده انگلیسی

A new simple immunoassay method for carcinoembryonic antigen (CEA) detection using a disposable immunosensor coupled with a flow injection system was developed. The immunosensor was prepared by coating CEA/colloid Au/chitosan membrane at a screen-printed carbon electrode (SPCE). Using a competitive immunoassay format, the immunosensor inserted in the flow system with an injection of sample and horseradish peroxidase (HRP)-labeled CEA antibody was used to trap the labeled antibody at room temperature for 35 min. The current response obtained from the labeled HRP to thionine–H2O2 system decreased proportionally to the CEA concentration in the range of 0.50–25 ng/ml with a correlation coefficient of 0.9981 and a detection limit of 0.22 ng/ml (S/N = 3). The immunoassay system could automatically control the incubation, washing and current measurement steps with good stability and acceptable accuracy. Thus, the proposed method proved its potential use in clinical immunoassay of CEA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 22, Issue 1, 15 July 2006, Pages 102–108
نویسندگان
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