Effect of oregano essential oil and carvacrol on Cryptosporidium parvum infectivity in HCT-8 cells

Schematic of in vitro assessment of C. parvum growth and invasion. In modality 1, oocysts and excysting sporozoites are exposed to different dosages of OEO or CV for 4 h. Then, 2-day incubation with untreated media. In modality 2, sporozoites (trophozoites) attached or permeated in HCT-8 cell monolayers in the first 4 h and undergoing asexual division into merozoites are exposed to different dosages of bioactive for 2 days.170