کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8855256 | 1619014 | 2018 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Selective concentration for ciprofloxacin resistance in Escherichia coli grown in complex aquatic bacterial biofilms
ترجمه فارسی عنوان
غلظت انتخابی برای مقاومت به سایپروفلوکساسین در اشرشیاکلی کشت شده در بیوفیلم های باکتری های پیچیده آبی
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کلمات کلیدی
WWTPMICMSCNOECLOECQRDRArg - ارگWastewater treatment plant - تصفیه خانه فاضلاب Minimal inhibitory concentration - حداقل غلظت مهار کنندهNo effect concentration - غلظت اثر نداردAntibiotic resistance - مقاومت به آنتی بیوتیکQuinolone resistance-determining region - منطقه تعیین کننده مقاومت کوئینولونAntibiotic resistance gene - ژن مقاومت آنتی بیوتیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
چکیده انگلیسی
There is concern that antibiotics in the environment can select for and enrich bacteria carrying acquired antibiotic resistance genes, thus increasing the potential of those genes to emerge in a clinical context. A critical question for understanding and managing such risks is what levels of antibiotics are needed to select for resistance in complex bacterial communities. Here, we address this question by examining the phenotypic and genotypic profiles of aquatic communities exposed to ciprofloxacin, also evaluating the within-species selection of resistant E. coli in complex communities. The taxonomic composition was significantly altered at ciprofloxacin exposure concentrations down to 1â¯Î¼g/L. Shotgun metagenomic analysis indicated that mobile quinolone resistance determinants (qnrD, qnrS and qnrB) were enriched as a direct consequence of ciprofloxacin exposure from 1â¯Î¼g/L or higher. Only at 5-10â¯Î¼g/L resistant E.coli increased relative to their sensitive counterparts. These resistant E. coli predominantly harbored non-transferrable, chromosomal triple mutations (gyrA S83â¯L, D87N and parC S80I), which confer high-level resistance. In a controlled experimental setup such as this, we interpret effects on taxonomic composition and enrichment of mobile quinolone resistance genes as relevant indicators of risk. Hence, the lowest observed effect concentration for resistance selection in complex communities by ciprofloxacin was 1â¯Î¼g/L and the corresponding no observed effect concentration 0.1â¯Î¼g/L. These findings can be used to define and implement discharge or surface water limits to reduce risks for selection of antibiotic resistance in the environment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environment International - Volume 116, July 2018, Pages 255-268
Journal: Environment International - Volume 116, July 2018, Pages 255-268
نویسندگان
Nadine Kraupner, Stefan Ebmeyer, Johan Bengtsson-Palme, Jerker Fick, Erik Kristiansson, Carl-Fredrik Flach, D.G. Joakim Larsson,