کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8887592 1628246 2018 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Development and characterization of 43 microsatellite markers for the critically endangered primrose Primula reinii using MiSeq sequencing
چکیده انگلیسی
Primula reinii (Primulaceae), a perennial herb belonging to the Primula section Reinii, occurs on wet, shaded rocky cliffs in the mountains of Japan. This threatened species comprises four varieties; these plants are very localized and rare in the wild. In this study, 43 microsatellite markers were developed using MiSeq sequencing to facilitate conservation genetics of these critically endangered primroses. We developed novel microsatellite markers for three varieties of P. reinii, and tested its polymorphism and genetic diversity using natural populations. These novel markers displayed relatively high polymorphism; the number of alleles and expected heterozygosities ranged from 2 to 6 (mean = 3.2) and 0.13 to 0.82 (mean = 0.45), respectively. All loci were in Hardy-Weinberg equilibrium. These microsatellite markers will be powerful tools to assess P. reinii genetic diversity and develop effective conservation and management strategies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Diversity - Volume 40, Issue 1, February 2018, Pages 41-44
نویسندگان
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