کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8887948 | 1628375 | 2018 | 40 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel bacteriocin DY4-2 produced by Lactobacillus plantarum from cutlassfish and its application as bio-preservative for the control of Pseudomonas fluorescens in fresh turbot (Scophthalmus maximus) fillets
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel bacteriocin DY4-2 was produced by strain DY4-2 which was isolated from cutlassfish (Trichiurus lepturus) and identified as Lactobacillus plantarum. This bacteriocin reached the maximum production at stationary phase after 24â¯h incubation. It was purified by ethyl acetate extraction followed by ultrafiltration, Sephadex G25 chromatography and high-performance liquid chromatography. The molecular mass of bacteriocin DY4-2 was determined as 1465â¯Da by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. The amino acid sequence of bacteriocin DY4-2 was predicted to be HAQIGMTMNGSFR by MALDI-TOF-MS/MS. Bacteriocin DY4-2 was heat-stable (30â¯minâ¯at 121â¯Â°C) and kept active at the pH range of 2.5-5.5, but was sensitive to proteolytic enzymes. The mode of action of bacteriocin DY4-2 was bactericidal. It exhibited broad spectrum antimicrobial activity against fish pathogenic and spoilage bacteria, such as Pseudomonas fluorescens, P. aeruginosa, Vibrio parahaemolyticus, Aeromonas sobria and Listeria monocytogenes. The addition of partially purified bacteriocin DY4-2 in turbot fillets reduced the number of P. fluorescens by 2.7 log units at 4â¯Â°C storage for 12 days. Moreover, compared to the control, a significant decline in total viable counts and total volatile base nitrogen was observed in bacteriocin DY4-2 treated samples. Theses results suggested that bacteriocin DY4-2 had potential to act as seafood bio-preservative.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 89, July 2018, Pages 22-31
Journal: Food Control - Volume 89, July 2018, Pages 22-31
نویسندگان
Xinran Lv, Huanhuan Ma, Mengtong Sun, Yang Lin, Fengling Bai, Jianrong Li, Bolin Zhang,