کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8949706 | 1645722 | 2018 | 46 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Desmoglein 3 - Influence on oral carcinoma cell migration and invasion
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کلمات کلیدی
OSCCDsg3LMAECMTME3D in vitro modelThree-dimensionalCAFNOFlow melting agarose - آگارز ذوب پایینImage analysis - تجزیه و تحلیل تصویرLive cell imaging - تصویربرداری سلول زندهInvasion - تهاجمdesmoglein 3 - دزوگلین 3two-dimensional - دو بعدیCell tracking - ردیابی سلولCarcinoma-associated fibroblast - فیبروبلاست وابسته به سرطانExtracellular matrix - ماتریکس خارج سلولیMigration - مهاجرتTumour microenvironment - میکرو محیط زیست تومورCo-culture - همکاری فرهنگیoral squamous cell carcinoma - کارسینوم سلول سنگفرشی دهانی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Desmoglein 3 (Dsg3) is an adhesion receptor in desmosomes, but its role in carcinoma cell migration and invasion is mostly unknown. Our aim was to quantitatively analyse the motion of Dsg3-modified carcinoma cells in 2D settings and in 3D within tumour microenvironment mimicking (TMEM) matrices. We tested mutant constructs of C-terminally truncated Dsg3 (â238 and â560), overexpressed full-length (FL) Dsg3, and empty vector control (Ct) of buccal mucosa squamous cell carcinoma (SqCC/Y1) cells. We captured live cell images and analysed migration velocities and accumulated and Euclidean distances. We compared rodent collagen and Matrigel® with human Myogel TMEM matrices for these parameters in 3D sandwich, in which we also tested the effects of monoclonal antibody AK23, which targets the EC1 domain of Dsg3. In monolayer culture, FL and both truncated constructs migrated faster and had higher accumulated distances than Ct cells. However, in the 3D assays, only the mutants invaded faster relative to Ct cells. Of the mutants, the shorter form (Î238) exhibited faster migration and invasion than Î560 cells. In the Transwell, all of the cells invaded faster through Myogel than Matrigel® coated wells. In 3D sandwich, AK23 antibody inhibited only the invasion of FL cells. We conclude that different experimental 2D and 3D settings can markedly influence the movement of oral carcinoma cells with various Dsg3 modifications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 370, Issue 2, 15 September 2018, Pages 353-364
Journal: Experimental Cell Research - Volume 370, Issue 2, 15 September 2018, Pages 353-364
نویسندگان
Ehsanul Hoque Apu, Saad Ullah Akram, Jouni Rissanen, Hong Wan, Tuula Salo,