کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8961168 | 1646472 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Separation of rotamers of 5-nitrosopyrimidines and estimation of binding constants of their complexes with β-cyclodextrin by capillary electrophoresis
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کلمات کلیدی
ACEEOFBGEHP-β-CDCM-β-CDDMSO - DMSOaffinity capillary electrophoresis - الکتروفورز مویرگی وابستهCapillary electrophoresis - الکتروفورزمویرگیbackground electrolyte - الکترولیت پس زمینهBinding constant - ثابت اتصالElectroosmotic flow - جریان الکترواسموتیکsupporting information - حمایت از اطلاعاتDegree of substitution - درجه جایگزینیDimethyl sulfoxide - دیمتیل سولفواکسیدCyclodextrin - سیکلودکسترینcyclodextrins - سیکلودکسترین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
For the first time, capillary electrophoresis has been successfully employed for the fast and highly efficient separations of a novel type of stereoisomers - planar rotamers (planamers) of four newly synthesized 5-nitrosopyrimidine derivatives. These derivatives can form two rotamers differing in the orientation of nitroso group due to strong intramolecular hydrogen bonds. Partial separation of rotamers of two 5-nitrosopyrimidines was achieved in alkaline 50âmM sodium tetraborate, pH 9.3, and in acidic 18.5/42âmM Tris/phosphate, pH 2.3, background electrolytes (BGEs) free of stereoselectors. To improve the separation of these rotamers and to attain the baseline or better separation of rotamers of other two 5-nitrosopyrimidines, various BGEs and different cyclodextrins-based stereoselectors were tested. The most effective, i.e. the fastest and baseline or better separations of rotamers of all analyzed 5-nitrosopyrimidines were achieved within a short time, 3.7-9.3âmin, in the above alkaline or acidic BGEs using β-cyclodextrin (β-CD) or carboxymethyl-β-CD as stereoselectors. Moreover, since the experiments with β-CD resulted in good separations of rotamers of all the investigated 5-nitrosopyrimidines, the apparent binding constants of their complexes with this selector were determined from the dependence of their effective mobilities on the β-CD concentration in the BGEs. The examined complexes were found to be relatively weak, with the apparent binding constants in the range 11.3-153.0âL/mol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1570, 5 October 2018, Pages 164-171
Journal: Journal of Chromatography A - Volume 1570, 5 October 2018, Pages 164-171
نویسندگان
Sille Å tÄpánová, EliÅ¡ka Procházková, Lucie Äechová, JiÅà Žurek, Zlatko Janeba, Martin DraÄÃnský, Václav KaÅ¡iÄka,