کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8965649 | 1646730 | 2018 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
C190A knockdown mutation in sodium channel domain II of pyrethroid-resistant Rhipicephalus appendiculatus
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP). A non-synonymous mutation C78âA corresponding to C190âA was found in all the five SP-resistant ticks. The C78âA mutation led to amino acid substitution from leucine to isoleucine (L21I) which was previously reported to confer knockdown (kdr) mutation in R. (Boophilus) microplus. The genetic confirmation of SP-resistant R. appendiculatus in central and southwestern Uganda calls for an urgent strategy for controlling the ticks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ticks and Tick-borne Diseases - Volume 9, Issue 6, September 2018, Pages 1590-1593
Journal: Ticks and Tick-borne Diseases - Volume 9, Issue 6, September 2018, Pages 1590-1593
نویسندگان
Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, John Son Bbira, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki,