کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9002136 | 1118574 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stimulation of intracellular Ca2+ elevation in neutrophils by thiol-oxidizing phenylarsine oxide
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
MBBRSOCE2-APBphorbol 12-myristate 13-acetatePKCU-73122DMPSPLCIP3N-acetyl-l-cysteinePMCASKF-96365HBSSLY 294002PIK32-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-oneDMPNACDAF-2/DADTTROIPAOCPAtris-(2-carboxyethyl)phosphine1400W - 1400 وات2′,7′-dichlorodihydrofluorescein diacetate - 2 '، 7'-dichlorodihydrofluorescein diacetate4,5-diaminofluorescein diacetate - 4،5-دی آمین فلوئورسین دی سکتهd-myo-inositol 1,4,5-trisphosphate - D-myo-inositol 1،4،5-trisphosphatePMA - LDC هاArachidonic acid - اسید آراشیدونیکCyclopiazonic acid - اسید سیکلوپیاازونیکPhenylarsine oxide - اکسید فنیلارسینdithiothreitol - دیتیوتریتولsarco/endoplasmic reticulum Ca2+-ATPase - سارکو / سلولهای اندوپلاسمی Ca2 + -ATPaseTCEP - ساکتPlasma membrane Ca2+-ATPase - غشای پلاسما Ca2 + -ATPasephospholipase C - فسفولیپاز CSERCA - قلبHanks’ balanced salt solution - محلول نمک متعادل هانکسMonobromobimane - مونوبرموبیمنNeutrophils - نوتروفیل هاNitric oxide - نیتریک اکسیدSignal transduction - هدایت سیگنالReactive oxygen intermediates - واکنش اکسیژن واسطهStore-operated Ca2+ entry - ورودی Ca2 + ذخیره شدهProtein kinase C - پروتئین کیناز سی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Phenylarsine oxide (PAO), a trivalent arsenical compound, stimulated [Ca2+]i elevation in rat neutrophils in a Ca2+-containing medium but caused no appreciable response in a Ca2+-free medium. PAO also induced external Mn2+ entry, which was inhibited by N-acetyl-l-cysteine (NAC), but failed to elicit any appreciable Ba2+ and Sr2+ entry. Pretreatment of neutrophils with thiol-reducing agents including dithiothreitol (DTT), NAC, 2,3-dimercapto-1-propanol (DMP), 2,3-dimercaptopropane-1-sulfonic acid (DMPS) and tris-(2-carboxyethyl)phosphine (TCEP), all greatly inhibited PAO-induced [Ca2+]i elevation. Addition of Ni2+ or La3+ followed by PAO stimulation also attenuated the Ca2+ signals in a concentration-dependent manner. PAO had no significant effect on the production of reactive oxygen intermediates (ROI) and nitric oxide (NO) nor did it decrease cellular low molecular weight thiols levels. PAO-induced [Ca2+]i elevation was significantly inhibited by 1-[6-[17β-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione (U-73122), the inhibitor of phospholipase C-coupled processes, genistein, a general tyrosine kinase inhibitor, phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, calyculin A, a cortical actin stabilizer, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY 294002), a phosphoinositide 3-kinase inhibitor, 1-[β-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl]-1H-imidazole (SKF-96365), and cis-N-(2-phenylcyclopentyl)azacyclotridec-1-en-2-amine (MDL-12,330A), the blockers of receptor-gated and store-operated Ca2+ channels, whereas there was no appreciable effect exerted by aristolochic acid, a phospholipase A2 inhibitor, 7-nitroindazole and N-(3-aminomethyl)benzylacetamidine (1400W), the blockers of NO synthase, and by suspension in a Na+-deprived medium. In contrast, 2-aminoethoxydiphenyl borane (2-APB), the blocker of IP3 receptor and Ca2+ influx, enhanced the PAO-induced response. PAO had no effect on the plasma membrane Ca2+-ATPase (PMCA) activity in the pharmacological isolated neutrophil preparation and the neutrophil membrane fractions. These results indicate that PAO stimulates [Ca2+]i rise in rat neutrophils mainly through the oxidation of vicinal thiol groups on the cell surface membrane to activation of a non-store operated Ca2+ entry (non-SOCE) without affecting the activity of PMCA and the plasmalemmal Na+/Ca2+ exchanger.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 69, Issue 8, 15 April 2005, Pages 1225-1234
Journal: Biochemical Pharmacology - Volume 69, Issue 8, 15 April 2005, Pages 1225-1234
نویسندگان
Jih-Pyang Wang, Jaw-Ji Tsai, Yu-San Chen, Mei-Feng Hsu,