کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9030660 | 1130932 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dietary modulation of 7,12-dimethylbenz[a]anthracene (DMBA)-induced adrenal toxicity in female Sprague-Dawley rats
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کلمات کلیدی
DMBAAdrenal toxicityACTH, adrenocorticotropin - ACTH، آدرنوکورتیکوتروپینDAB, diaminobenzidine - DAB، diaminobenzidineDMBA, 7,12-dimethylbenz[a]anthracene - DMBA، 7،12-dimethylbenz [a] anthracenePND, postnatal day - PND، روز پس از زایمانer, estrogen receptor - است، گیرنده استروژنSD, standard deviation - انحراف معیارApoptosis - خزان یاختهایDiet - رژیم غذاییSoy - سویاCYP, cytochrome P450 - سیتوکروم P450H&E, Hematoxylin and Eosin - هماتوکسیلین و ائوزین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, dietary modulation of 7,12-dimethylbenz[a]anthracene (DMBA)-induced adrenal toxicity in rats was investigated. Beginning at postnatal day (PND) 21, female Sprague-Dawley rats were fed either soy-containing NIH-31 diet or soy- and alfalfa-free 5K96 diet. On the first day of diestrus when the animals were PND 50 ± 5, rats received either an oral dose of 80 mg/kg DMBA or sesame oil, the vehicle, and were sacrificed at 24, 36, or 48 h after treatment. Apoptosis was manifested at 24 and 36 h after DMBA treatment in the zona reticularis (ZR) and the zona fasciculata (ZF) of the adrenal cortex; this was followed by severe hemorrhagic necrosis at 48 h. DMBA-induced apoptosis, evaluated by the TUNEL assay, immunohistochemical analysis of activated caspase 3, and the ratio of expression of pro-apoptotic Bax to anti-apoptotic Bcl2, was greater in rats fed NIH-31 diet relative to rats fed 5K96 diet at 24 h after treatment. Four of six DMBA-treated rats fed 5K96 diet had severe adrenal necrosis by 48 h, whereas this lesion was present in only two of six DMBA-treated rats fed NIH-31 diet. DMBA also caused a significant decrease of serum corticosterone relative to controls at 48 h in rats fed 5K96 diet. The present study indicated that diet modulates DMBA-induced adrenal toxicity in female rats, with increased apoptosis early and reduced necrosis later in rats fed a soy-containing diet.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 43, Issue 5, May 2005, Pages 765-774
Journal: Food and Chemical Toxicology - Volume 43, Issue 5, May 2005, Pages 765-774
نویسندگان
Xin Fu, John R. Latendresse, Levan Muskhelishvili, Betty S. Blaydes, K. Barry Delclos,