کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9038428 | 1133832 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro cytotoxicity of selected chemicals commonly produced during fire combustion using human cell lines
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کلمات کلیدی
HSDBHbRLC50TLCIC50A549PMSMEICNTPLD50HClHepG2IC1050% inhibitory concentration - 50٪ غلظت مهاریDMEM/F12 - DMEM / F12KCN - IP هاMulticentre Evaluation of In Vitro Cytotoxicity - ارزیابی چند مرکز سیتوتوکسیتیک در داخل ویروسیISO - ایزوNational Toxicology Program - برنامه سم شناسی ملیanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceMTS assay - تست MTSHuman cell lines - خطوط سلولی انسانlethal dose 50% - دوز مرگبار 50٪Sulphur dioxide - دی اکسید گوگردSO2 - دی اکسید گوگردInternational Organization for Standardization - سازمان بین المللی استاندارد سازیIn vitro cytotoxicity - سمیت سلولی in vitrolethal concentration 50% - غلظت کشنده 50٪Hydrogen fluoride - فلوراید هیدروژنHydrogen bromide - هیدروژن برومیدpotassium cyanide - پتاسیم سیانیدHydrogen chloride - کلرید هیدروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Fire combustion products contain a broad range of chemicals, which have a multitude of possible toxic interactions in humans. The aim of this study was to evaluate the cytotoxicity of selected substances commonly produced during fire combustion. A range of human cell lines and cultures including: skin fibroblasts, HepG2 (liver derived), and A549 (lung derived cells) were used to represent different human target organs. The colorimetric MTS assay (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) was used to detect the cytotoxic effects of selected substances including: acetic acid, ammonia, formaldehyde, hydrobromic acid, hydrochloric acid, hydrofluoric acid, potassium cyanide, sodium fluoride, sodium nitrite, sodium sulphide, and sulphurous acid. In this study, the NOAEC (No Observable Adverse Effect Concentration), IC10 (10% inhibitory concentration), IC50 (50% inhibitory concentration), and TLC (Total Lethal Concentration) values were determined. The ratio between in vitro IC50 to in vivo human toxicity data (Lowest Lethal Dose-LDLo and Lowest Lethal Concentration--LCLo) was also established. Results indicated a strong relationship between IC50 values on the cell types used: fibroblast and A549 (R2: 0.92), A549 and HepG2 (R2: 0.72), fibroblast and HepG2 (R2: 0.69). Good correlation was obtained between the IC50 against LDLo and LCLo when an appropriate adjustment factor was implemented. Results of this study indicated that in vitro methods could be a potential technique for assessing the toxicity of fire combustion products.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 19, Issue 5, August 2005, Pages 653-663
Journal: Toxicology in Vitro - Volume 19, Issue 5, August 2005, Pages 653-663
نویسندگان
F. Lestari, A.J. Hayes, A.R. Green, B. Markovic,