کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9038821 | 1133873 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Toxicity of tire debris extracts on human lung cell line A549
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
TD, produced by tire wear, is a significant constituent of PM10 in urban areas where traffic related emissions are predominant. TD contains a lot of chemicals which can affect human respiratory system and it has received little attention until now, even the toxicity of PM has been extensively documented. A549 cells, a human alveolar lung cells, were exposed for 24, 48, 72 h to 10, 50, 60, 75 μg/ml of TD organic extract. MTT and Trypan Blue assays were used to evaluate cytotoxicity and Comet Assay to evidence DNA damage. TD extracts induced a dose-dependent increase in cell mortality and DNA damage. A significant toxicity was observed when cells were exposed to 60 μg/ml for 72 h. Moreover cell morphology observed at ultra structural level, was severely affected at the highest dose.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 19, Issue 7, October 2005, Pages 1001-1008
Journal: Toxicology in Vitro - Volume 19, Issue 7, October 2005, Pages 1001-1008
نویسندگان
Maurizio Gualtieri, Laura Rigamonti, Valentina Galeotti, Marina Camatini,