کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127242 | 1160205 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation
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کلمات کلیدی
AP-1UTRrRNAreverse-transcriptaseMTF-1terminal deoxynucleotide transferaseMRETdTGSPTPENq-PCR - Q-PCRRibosomal RNA - RNA RibosomalSDS–PAGE - SDS-PAGEgene specific primer - آغازگر مخصوص ژنEDTA - اتیلن دی آمین تترا استیک اسید ethylenediamine tetraacetic acid - اسید اتیلنیدین تتراستیکGene expression - بیان ژنrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدcoding sequence - توالی کدگذاریbase pair - جفت پایهReal time quantitative PCR - زمان واقعی PCR کمیMussels - صدفmetal responsive element - عنصر پاسخگو فلزیMetallothionein - متالوتونیئینRace - مسابقهuntranslated region - منطقه غیر ترجمهNucleotide - نوکلئوتیدpolymerase chain reaction - واکنش زنجیره ای پلیمرازquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPCR - واکنش زنجیرهٔ پلیمرازactivator protein-1 - پروتئین فعال کننده-1threshold cycle - چرخه آستانهCdS - کادمیم سولفید، سولفید کادمیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation](/preview/png/9127242.png)
چکیده انگلیسی
The mRNA levels of two components of the mussel (Mytilus galloprovincialis) metallothionein (MT) gene families, MT10 and MT20, were evaluated using real-time quantitative-PCR and Sybr Green I chemistry in animals exposed to heavy metals in vivo and in primary cell cultures. This method was highly specific in detecting the expression of the two genes over a widely dynamic range of starting DNA amounts, showing that the basal level of MT expression is mostly due to MT10 mRNA. Basal MT expression reflected the intracellular concentration of heavy metal as indicated by the use of the heavy metal chelator TPEN on primary cells. MT10 was observed to be inducible by Cd, Zn, and Cu ions, and to a lesser extent by Hg. By contrast, the MT20 expression level was very low under basal conditions, while its mRNA increased dramatically in response to Cd exposure, and to a lesser extend to Hg, leading to levels of expression similar to those of the MT10 gene. The essential metals Cu and Zn had a very small effect on the MT20 gene, whereas the concomitant exposure to Cu and H2O2 produced a rapid rise of expression. In summary, data indicate that the MT isogenes are differentially regulated by heavy metals, while hydroxyl radicals may have a role in MT20 gene activation. Also, protein expression showed metal inducibility only after Cd exposure, suggesting the occurrence of posttranscriptional control mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 345, Issue 2, 31 January 2005, Pages 259-270
Journal: Gene - Volume 345, Issue 2, 31 January 2005, Pages 259-270
نویسندگان
Francesco Dondero, Luciana Piacentini, Mohamed Banni, Mauro Rebelo, Bruno Burlando, Aldo Viarengo,