کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9192144 | 1186615 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulated sarcolemmal localization of the muscle-specific ClC-1 chloride channel
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کلمات کلیدی
EDLClC-1DHPRMyotoniaSFVBHKADRFDBPBSα-bungarotoxinPMSFPAGESDSBTXBSA - BSAbovine serum albumin - آلبومین سرم گاوEDTA - اتیلن دی آمین تترا استیک اسید ethylene diamine tetra-acetic acid - اتیلن دیامین تترا استیک اسیدpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدbaby hamster kidney - بچه هامستر کلیهsodium dodecyl sulfate - سدیم دودسیل سولفاتSol - سلMyotonia congenita - سندرم متابولیکendoplasmic reticulum - شبکه آندوپلاسمی Phosphate buffered saline - فسفات بافر شورPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدmyofiber - میوفیببرSemliki Forest virus - ویروس Semliki جنگلChloride channel - کانال کلرایدdihydropyridine receptor - گیرنده dihydropyridine
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The skeletal muscle-specific ClC-1 is a voltage-gated chloride channel protein. Specific antibodies against ClC-1 revealed in muscle sections a sarcolemmal staining that was absent in the myotonic arrested development of righting response (ADR) mouse muscle. The intensity of the sarcolemmal staining varied from one type of muscle to another and in lateral sections showed a typical mosaic pattern that colocalized with β-dystroglycan and left the transverse tubule openings clear. Surprisingly, in isolated myofibers, the ClC-1 protein was absent from the sarcolemma. Instead, it localized to intracellular I band areas as soon as the myofibers were isolated. When the isolated myofibers were incubated with the kinase inhibitor staurosporine, the ClC-1 protein shifted back to the sarcolemma. Electric stimulation of the cultivated fibers had a similar effect. Also, myofibers infected with a recombinant Semliki Forest virus (SFV) expressing myc-tagged ClC-1 showed intracellular localization of the protein. The virally expressed mycClC-1 reached the Golgi apparatus but sarcolemmal staining remained nondetectable, and addition of staurosporine into the growth medium recruited the mycClC-1 to the sarcolemma. These data indicate that sarcolemmal targeting of the ClC-1 requires specific signals that are provided by the physiological environment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Neurology - Volume 191, Issue 1, January 2005, Pages 163-173
Journal: Experimental Neurology - Volume 191, Issue 1, January 2005, Pages 163-173
نویسندگان
H. Papponen, T. Kaisto, V.V. Myllylä, R. Myllylä, K. Metsikkö,