کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9279430 1593515 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Oligonucleotide-based microarray: A new improvement in microarray detection of plant viruses
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Oligonucleotide-based microarray: A new improvement in microarray detection of plant viruses
چکیده انگلیسی
Microarrays are one of the new emerging methods in plant virology currently being developed by various laboratories. In this study, a new approach is described on the detection of plant viruses using short synthetic single-stranded oligomers (40 nt) instead of PCR products as capture probes. A microchip detecting potato viruses, PVA, PVS, PVM, PVX, PVY and PLRV, in both single and mixed infections was developed and tested. The chip was also designed to distinguish between the main strains of PVY and PVS. Results of initial tests with PVYNTN and PVYO strains using several different probes for one virus are presented. Possibilities and advantages of the new oligonucleotide-based microarray approach for plant viral diagnosis are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 128, Issues 1–2, September 2005, Pages 176-182
نویسندگان
, , , , , ,