کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9282883 | 1593662 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional analysis of immune response genes in Drosophila identifies JNK pathway as a regulator of antimicrobial peptide gene expression in S2 cells
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کلمات کلیدی
AOPTNFGNBPPAMPMSNPRRIMDHEPMisshapenPGRPMAPK - MAPKInnate immunity - ایمنی ذاتیJNK signaling - سیگنالینگ JNKtumor necrosis factor - فاکتور نکروز تومورDrosophila melanogaster - مگس سرکه، مگس میوهhemipterous - همرنگpathogen associated molecular pattern - پاتوژن الگوی مولکولی مرتبط استPeptidoglycan recognition protein - پروتئین شناخته شده Peptidoglycanmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenkayak - کایاکpattern recognition receptor - گیرنده شناسایی الگو
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Functional analysis of immune response genes in Drosophila identifies JNK pathway as a regulator of antimicrobial peptide gene expression in S2 cells Functional analysis of immune response genes in Drosophila identifies JNK pathway as a regulator of antimicrobial peptide gene expression in S2 cells](/preview/png/9282883.png)
چکیده انگلیسی
The templates of innate immunity have ancient origins. Thus, such model animals as the fruit fly, Drosophila melanogaster, can be used to identify gene products that also play a key role in the innate immunity in mammals. We have used oligonucleotide microarrays to identify genes that are responsive to Gram-negative bacteria in Drosophila macrophage-like S2 cells. In total, 53 genes were induced by greater than threefold in response to Escherichia coli. The induction of all these genes was peptidoglycan recognition protein LC (PGRP-LC) dependent. Twenty-two genes including 10 of the most strongly induced genes are also known to be up-regulated by septic injury in vivo. Importantly, we identified 31 genes that are not known to respond to bacterial challenge. We carried out targeted dsRNA treatments to assess the functional importance of these gene products for microbial recognition, phagocytosis and antimicrobial peptide release in Drosophila S2 cells in vitro. RNAi targeting three of these genes, CG7097, CG15678 and β-Tubulin60D, caused altered antimicrobial peptide release in vitro. Our results indicate that the JNK pathway is essential for normal antimicrobial peptide release in Drosophila in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 7, Issues 5â6, May 2005, Pages 811-819
Journal: Microbes and Infection - Volume 7, Issues 5â6, May 2005, Pages 811-819
نویسندگان
Jenni Kallio, Anni Leinonen, Johanna Ulvila, Susanna Valanne, R. Alan Ezekowitz, Mika Rämet,