کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9282933 | 1224994 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Efficacy of cell-free antigens in evaluating cell immunity and inducing protection in a murine model of histoplasmosis
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Efficacy of cell-free antigens in evaluating cell immunity and inducing protection in a murine model of histoplasmosis Efficacy of cell-free antigens in evaluating cell immunity and inducing protection in a murine model of histoplasmosis](/preview/png/9282933.png)
چکیده انگلیسی
Histoplasma capsulatum is a dimorphic pathogenic fungus that causes a wide spectrum of disease when mycelial fragments are inhaled. Resistance to H. capsulatum is dependent on cellular immunity mediated by T cells and macrophages. Here we standardized the production of extracts containing cell-free antigens (CFAgs) and observed their efficacy in evaluating cellular immunity during murine histoplasmosis. CFAgs induced a more potent delayed-type hypersensitivity (DTH) response in H. capsulatum-infected mice than did histoplasmin-a classical antigen. This DTH response to CFAgs is able to determine the immune status of infected mice and to predict their death. Moreover, CFAgs stimulated spleen cells from immune mice to produce higher amounts of gamma interferon (IFN-γ) in vitro. Finally, immunization with CFAgs protected against a lethal inoculum of H. capsulatum. These results demonstrate that CFAgs may be useful for the evaluation of cellular immune response and as a potential source for the development of a vaccine against histoplasmosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 7, Issue 4, April 2005, Pages 584-592
Journal: Microbes and Infection - Volume 7, Issue 4, April 2005, Pages 584-592
نویسندگان
Anderson Sá-Nunes, Alexandra I. Medeiros, Roberto Nicolete, Fabiani G. Frantz, Ademilson Panunto-Castelo, Célio L. Silva, Lúcia H. Faccioli,