کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9282967 | 1224995 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The varicella-zoster virus-mediated delayed host shutoff: open reading frame 17 has no major function, whereas immediate-early 63 protein represses heterologous gene expression
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PAAVHSp.i.NEAAPDHBHV-1EHV-1VZVβ-galEtBrFCSRT-PCRPRVORFGAPDHHSVP/S - P / Sethidium bromide - اتیدیوم برومایدnon-essential amino acids - اسید آمینه غیر ضروریPhosphonoacetic acid - اسید فسفونوئیکسβ-galactosidase - بتا گالاکتوزیدازReporter gene expression - بیان ژن خبرنگارfetal calf serum - سرم گوساله جنینopen reading frame - قاب خواندن بازEquine herpesvirus type 1 - نوع هرپس ویروس اسب 1reverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسPseudorabies virus - ویروس Pseudorabiesherpes simplex virus - ویروس هرپس سیمپلکسVaricella–zoster virus - ویروس واریلا زوسترBovine herpesvirus type 1 - ویروس گاو نوع 1post-infection - پس از عفونتPenicillin/streptomycin - پنیسیلین / استرپتومایسینpyruvate dehydrogenase - پیرووات دهیدروژنازLate gene - ژن بعدEarly gene - ژن زودرسImmediate–early gene - ژن فوریglyceraldehydes-3-phosphate dehydrogenase - گلیسرالید هیدروژن 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We reported that varicella-zoster virus (VZV) causes a delayed host shutoff during its replicative cycle. VZV open reading frame 17 (ORF17) is the homologue of the herpes simplex virus (HSV) UL41 gene encoding the virion host shutoff (vhs) protein which is responsible for the shutoff effect observed in HSV-infected cells. In the present study, we demonstrated that ORF17 is expressed as a late protein during the VZV replicative cycle in different infected permissive cell lines which showed a delayed shutoff of cellular RNA. A cell line with stable expression of VZV ORF17 was infected with VZV. In these cells, VZV replication and delayed host shutoff remained unchanged when compared to normal infected cells. ORF17 was not capable of repressing the expression of the β-gal reporter gene under the control of the human cytomegalovirus immediate-early gene promoter or to inhibit the expression of a CAT reporter gene under the control of the human GAPDH promoter, indicating that ORF17 has no major function in the VZV-mediated delayed host shutoff. To determine whether other viral factors are involved in the host shutoff, a series of cotransfection assays was performed. We found that the immediate-early 63 protein (IE63) was able to downregulate the expression of reporter genes under the control of the two heterologous promoters, indicating that this viral factor can be involved in the VZV-mediated delayed host shutoff. Other factors can be also implicated to modulate the repressing action of IE63 to achieve a precise balance between the viral and cellular gene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 7, Issue 15, December 2005, Pages 1519-1529
Journal: Microbes and Infection - Volume 7, Issue 15, December 2005, Pages 1519-1529
نویسندگان
Nathalie Desloges, Markus Rahaus, Manfred H. Wolff,