کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9286893 | 1227380 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of clathrin-mediated endocytosis during vesicular stomatitis virus entry into host cells
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کلمات کلیدی
EGFCCVGFPVSVSFVSmall interfering RNA - RNA تداخل کوچکRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsiRNA - siRNAEndocytosis - آندوسیتوز یا درون بریepidermal growth factor - عامل رشد اپیدرمیVirus entry - ورود ویروسSemliki Forest virus - ویروس Semliki جنگلVesicular stomatitis virus - ویروس vesicular stomatitisgreen fluorescent protein - پروتئین فلورسنت سبزClathrin - کلاتینclathrin-coated vesicle - کیسه کلاسیک کلاسیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Vesicular stomatitis virus (VSV) is well established to enter cells by pH-dependent endocytosis, but mechanistic aspects of its internalization have remained unclear. Here, we examined the functional role of clathrin in VSV entry by expression of a dominant-negative mutant of Eps15 (GFP-Eps15Î95/295), a protein essential for clathrin-mediated endocytosis. Whereas expression of GFP alone had no effect on VSV infection, expression of GFP-Eps15Î95/295 severely limited infection. As independent ways to examine clathrin function, we also examined cells that had been treated with chlorpromazine and utilized small interfering RNA (siRNA) techniques. Inhibition of clathrin-mediated endocytosis by chlorpromazine treatment, as well as clathrin knock-down using siRNA duplexes directed against the clathrin heavy chain, also prevented VSV infection. In combination with previous morphological approaches, these experiments establish clathrin as an essential component needed for endocytosis of VSV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 338, Issue 1, 20 July 2005, Pages 53-60
Journal: Virology - Volume 338, Issue 1, 20 July 2005, Pages 53-60
نویسندگان
Xiangjie Sun, Vivian K. Yau, Benjamin J. Briggs, Gary R. Whittaker,