کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604236 | 43608 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We raised monoclonal antibodies by immunizing mice with total chromosome proteins extracted from isolated human metaphase chromosomes. The indirect immunofluorescence screening of hybridoma cell lines provided 15 monoclonal antibodies against the chromosomal antigens. The antigen proteins of the mAbs were identified by immunoblotting as core histones or by immunoprecipitation followed by a peptide mass fingerprinting method as nuclear mitotic apparatus protein, heterogeneous nuclear ribonucleoprotein A2/B1, ribosomal protein S4, linker histone and β-actin. During mitosis, localizations of these proteins on chromosomes were clearly observed using the obtained antibodies. These results indicate that the current strategy is effective for obtaining monoclonal antibodies useful for immunoblotting and/or immunofluorescent staining of human proteins, using the antigens with high homology to mouse proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 120, Issue 3, 21 November 2005, Pages 262-272
Journal: Journal of Biotechnology - Volume 120, Issue 3, 21 November 2005, Pages 262-272
نویسندگان
Tsunehito Higashi, Shuichi Miyakawa, Susumu Uchiyama, Sachihiro Matsunaga, Hideaki Takata, Satoru Fujimoto, Masanori Noda, Akiko Terauchi, Takeyuki Shimizu, Masayuki Oda, Takachika Azuma, Kiichi Fukui,