کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604262 | 43610 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of flow-cytometric analysis to optimize cell banking strategies for production of biopharmaceuticals from mammalian cells
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Use of flow-cytometric analysis to optimize cell banking strategies for production of biopharmaceuticals from mammalian cells Use of flow-cytometric analysis to optimize cell banking strategies for production of biopharmaceuticals from mammalian cells](/preview/png/9604262.png)
چکیده انگلیسی
Production of biopharmaceuticals from mammalian cells requires generation of master, working and post-production cell banks of high quality under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to establishing production processes that are completely devoid of serum or even any animal components and to ensuring robust thaw performance for reliable production. Here, we describe a novel strategy employing flow-cytometric (FC) analysis of Annexin V-stained cells for high-throughput characterization of cell banks. Our data show that this method enables predictive evaluation of a cryopreservation strategy as early as 6Â h after thawing of cells. Furthermore, a broad study is presented characterizing various factors that may influence the quality of serum-free production cell banks from NSO and CHO cell lines. These results demonstrate how FC-based analysis can be used for development of future state-of-the-art cryopreservation strategies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 120, Issue 1, 17 October 2005, Pages 111-120
Journal: Journal of Biotechnology - Volume 120, Issue 1, 17 October 2005, Pages 111-120
نویسندگان
Jürgen Fieder, Nina Wagner, Stefanos Grammatikos, Helmut Hoffmann, Hitto Kaufmann, Ralf T. Otto,