کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604324 | 43611 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of glucuronan oligosaccharides using a new glucuronan lyase activity from a Trichoderma sp. strain
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Sinorhizobium meliloti M5N1CS synthesizes a homopolymer of glucuronic acids β-(1,4) linked and variably C2 and/or C3O-acetylated. To obtain β-Î-(4,5)-unsaturated oligoglucuronans, various acetylated forms of this bacterial polymer were cleaved by a Trichoderma sp. GL2 glucuronan lyase. Oligomers with polymerization degrees up to 8 were then produced, purified by liquid chromatography (size exclusion and anions exchange) and characterized using 1H NMR and ESI-Q/TOF-MS. Finally, the production (in gram quantity) of pure unsaturated oligoglucuronans non-acetylated (di- and trisaccharide) was investigated thanks to the complete depolymerization of deacetylated glucuronan.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 118, Issue 4, 10 September 2005, Pages 448-457
Journal: Journal of Biotechnology - Volume 118, Issue 4, 10 September 2005, Pages 448-457
نویسندگان
C. Delattre, P. Michaud, J.M. Lion, B. Courtois, J. Courtois,