کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604359 | 43600 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Practical resolution system for dl-pantoyl lactone using the lactonase from Fusarium oxysporum
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The lactonase catalyzes the stereospecific hydrolysis of d-pantoyl lactone. One hundred eighty repeated batch reactions (total reaction time, 3780 h) were made with mycelia entrapped in calcium alginate gels as the catalyst, in the presence of 90 mM CaCl2. With a 300 g lâ1dl-pantoyl lactone solution as the substrate, the hydrolysis rate for dl-pantoyl lactone was >40% and the optical purity of d-pantoic acid was 90% enantiomer excess. Immobilized mycelia retained 70% of their initial lactonase activity, even after 180 batch reactions. The estimated half-life of the lactonase activity of the immobilized mycelia was 6000 h, which is 35 times higher than that of the free mycelia. The process has been exploited commercially since 1999.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 118, Issue 1, 2 July 2005, Pages 99-106
Journal: Journal of Biotechnology - Volume 118, Issue 1, 2 July 2005, Pages 99-106
نویسندگان
Keiji Sakamoto, Kohsuke Honda, Koichi Wada, Shinji Kita, Kazuya Tsuzaki, Hanae Nose, Michihiko Kataoka, Sakayu Shimizu,