کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9604406 | 43633 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alkali and halide-resistant catalysis by the multipotent oxidase from Marinomonas mediterranea
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
The incorporation of fungal laccases into novel applications has been delayed mainly due to their intrinsic sensitivity towards halides and alkaline conditions. In order to explore new sources of enzymes we evaluated the multipotent polyphenol oxidase PPO1 from the marine bacterium Marinomonas mediterranea. Here we report that, in contrast to its fungal counterparts, PPO1 remained functional above neutral pH presenting high specificity for phenolic compounds, in particular for methoxyl-substituted mono-phenols and catechols. These properties, in addition to its tolerance towards chloride (up to 1Â M) and its elevated redox potential at neutral pH (0.9Â V), suggest this enzyme may be an interesting candidate for specific applications such as the Amperometric determination of phenolic compounds and bio-fuel cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 117, Issue 1, 20 April 2005, Pages 73-82
Journal: Journal of Biotechnology - Volume 117, Issue 1, 20 April 2005, Pages 73-82
نویسندگان
Nuria Jimenez-Juarez, Rosa Roman-Miranda, Alejandro Baeza, Antonio Sánchez-Amat, Rafael Vazquez-Duhalt, Brenda Valderrama,