کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9607019 44586 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorometric investigation of the interaction of bovine serum albumin with surfactants and 6-mercaptopurine
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Fluorometric investigation of the interaction of bovine serum albumin with surfactants and 6-mercaptopurine
چکیده انگلیسی
Fluorescence quenching in solutions of bovine serum albumin has been investigated in the presence of 6-mercaptopurine and ionic surfactants. Spectroscopic analysis of the emission quenching at different temperatures revealed that the quenching mechanism of bovine serum albumin by 6-mercaptopurine was dynamic quenching mechanism. The Stern-Volmer quenching model has been successfully applied, and the activation energy of the interaction between 6-mercaptopurine and bovine serum albumin as much as 4.26 kJ mol−1 was calculated. The distance r between donor (bovine serum albumin) and acceptor (6-mercaptopurine) was obtained according to fluorescence resonance energy transfer (FRET). The result of synchronous fluorescence spectra shows that the conformation of bovine serum albumin has been changed at the present of 6-mercaptopurine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 80, Issue 3, 1 September 2005, Pages 235-242
نویسندگان
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