کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9607090 | 44531 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Flow-cytometric monitoring of mitochondrial depolarisation: from fluorescence intensities to millivolts
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Flow-cytometric monitoring of mitochondrial depolarisation: from fluorescence intensities to millivolts Flow-cytometric monitoring of mitochondrial depolarisation: from fluorescence intensities to millivolts](/preview/png/9607090.png)
چکیده انگلیسی
Redistribution potentiometric dyes represent a powerful tool for monitoring membrane potential of mitochondria, especially when these dyes are used with flow cytometry. In particular, tetramethylrhodamine methyl ester proved to be suitable for the screening of mitochondrial membrane potential in cultured human skin fibroblasts from patients suffering from different defects of oxidative phosphorylation. We have developed a method that makes it possible to measure the changes in mitochondrial membrane potential, or to assess the differences between respective mitochondrial membrane potentials in investigated cells and controls in the absolute scale of millivolts. Our approach employs the fact that a logarithmic transformation of Nernst equation-controlled intensity of fluorescence from potentiometric dyes accumulated in mitochondria leads to a linear scale for mitochondrial membrane potentials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 78, Issue 2, 1 February 2005, Pages 99-108
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 78, Issue 2, 1 February 2005, Pages 99-108
نویسندگان
J. PláÅ¡ek, A. VojtÃÅ¡ková, J. HouÅ¡tÄk,