کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9616699 | 48915 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Revisit of aminotransferase in the genomic era and its application to biocatalysis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
کاتالیزور
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Aminotransferases (ATs) have useful applications in the chemical industry because of their capability of introducing amino group into ketones or keto acids as well as their high enantioselectivity and regioselectivity and broad substrate specificity. Abundant protein sequence databases and new powerful tools such as advanced computational structure modeling, multiple sequence analysis, and in vitro evolution have made it possible to understand the detailed reaction mechanisms of various ATs and to isolate and design novel enzymes for unnatural substrates. This, in turn, suggests that developing new integrated approaches to screen ATs are possible, but at the same time poses formidable technical challenges. Here, this paper reviews the use of family profile analysis to find the correlation between the type of ATs and their substrate specificities, the relation between the 3-D structures of ATs and their substrate specificities, and enzyme engineering for the synthesis of unnatural substrates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 37, Issues 1â6, 1 December 2005, Pages 47-55
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 37, Issues 1â6, 1 December 2005, Pages 47-55
نویسندگان
Bum-Yeol Hwang, Byung-Kwan Cho, Hyungdon Yun, Kinera Koteshwar, Byung-Gee Kim,