کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9616714 | 48872 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enantioselective production of halohydrin precursor of Propranolol catalysed by immobilized yeasts
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
کاتالیزور
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enantioselective production of halohydrin precursor of Propranolol catalysed by immobilized yeasts Enantioselective production of halohydrin precursor of Propranolol catalysed by immobilized yeasts](/preview/png/9616714.png)
چکیده انگلیسی
Two active yeasts for reduction of ketones have been immobilized in calcium alginate. The maximum productivity of alcohol dehydrogenases is achieved at the beginning of the stationary phase for Yarrowia lipolytica (48 h) and in the logarithmic phase in Pichia mexicana (12 h). The immobilization experimental conditions have been optimized by factorial experimental design. The influence of the volume of sodium alginate, alginate percentage, cell loading and amount of ketone has been studied. The immobilization slightly reduces the enzymatic activity in the case of P. mexicana compared to resting cells conditions whereas Y. lipolytica maintains the activity. Nevertheless, the immobilized biocatalysts can be reused, at least five times in the reduction of ketones. P. mexicana carries to the R-halohydrin (precursor of S-adrenergic β-blocker, Propranolol) with 80% yield and 96% enantiomeric excess (e.e.). Y. lipolytica carries to S-enantiomer of the halohydrin with 80% yield and e.e. 95-97%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 36, Issues 1â6, 1 November 2005, Pages 1-7
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 36, Issues 1â6, 1 November 2005, Pages 1-7
نویسندگان
F. MartÃnez-Lagos, J.V. Sinisterra,