کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9616749 48920 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of salts on activity, stability and enantioselectivity of Candida rugosa lipase in isooctane
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Effect of salts on activity, stability and enantioselectivity of Candida rugosa lipase in isooctane
چکیده انگلیسی
The effect of salts on activity and stability and enantioselectivity of Candida rugosa lipase in isooctane was studied. The activity of the lipase lyophilized with LiCl, NaCl or KCl and native lipase for the esterification of lauric acid with 1-propanol in isooctane at aw = 0.33 was 0.057, 0.044, 0.033 and 0.027 g g−1 min−1, respectively. The water content played a main role on the effect of lipase activity and lyophilization process did not change the conformation of the lipase. Salt incorporation could keep the conformation of the lipase and prevented the large change of optimum pH condition happening. The stability of the lyophilized lipase was 3.4 times as much as that of native lipase and the lipase thermostability was also improved. The enantioselectivity of lyophilized lipase (20.3) was about 1.6 times as much as that of the native lipase (13.0).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 35, Issues 1–3, 1 August 2005, Pages 28-32
نویسندگان
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