کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9616802 | 48895 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biocatalytic properties of native and immobilized subtilisin 72 in aqueous-organic and low water media
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
کاتالیزور
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Biocatalytic properties of native and immobilized subtilisin 72 in aqueous-organic and low water media Biocatalytic properties of native and immobilized subtilisin 72 in aqueous-organic and low water media](/preview/png/9616802.png)
چکیده انگلیسی
Subtilisin 72 was immobilized on cryogel of poly(vinyl alcohol), the macroporous carrier prepared by the freeze-thaw-treatment of concentrated aqueous solution of the polymer. The obtained biocatalyst was active and stable in aqueous, aqueous-organic, as well as in low water media. The stability of immobilized biocatalyst was substantially higher than that of native enzyme in all mixtures especially in aqueous buffer containing 5-8Â M Urea and in acetonitrile/60-90%DMF mixtures. The ability of native and immobilized subtilisin to catalyze peptide bond formation between Z-Ala-Ala-Leu-OMe and Phe-pNA was studied in non-aqueous media. Considerable enzyme stabilization in acetonitrile/90%DMF mixture, induced by the immobilization, resulted in higher product yield (57%) than in case of native subtilisin suspension (32%). Detailed study of synthesis reaction revealed that notable increase in product yield could be reached using increase in both substrate concentrations up to 200Â mM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 32, Issues 5â6, 1 March 2005, Pages 253-260
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 32, Issues 5â6, 1 March 2005, Pages 253-260
نویسندگان
Anna V. Bacheva, Anastasiya V. Belyaeva, Elena N. Lysogorskaya, Elena S. Oksenoit, Vladimir I. Lozinsky, Irina Yu. Filippova,