HPLC-DAD for analysis of different classes of drugs in plasma

A solid-phase extraction technique and HPLC-DAD method for drug screening in plasma were developed. This analytical method extracts and tests a number of drugs of different classes. The method includes; an acidic and basic Solid Phase Extraction (SPE) of plasma with C18 cartridges, a gradient elution of a modified cyano column with acidic buffer/acetonitrile eluent and a photodiode array ultraviolet (UV) detection. The drug screening procedure applied uses retention index and UV spectral data for the identification of compounds, may be appropriate in particular laboratory settings.