Investigation of RuBPS-Ce(IV) chemiluminescence reaction and its application in determination of two diuretics

The chemiluminescence mechanism of tris-(4,7-diphenyl-1,10-phenanthrolinedisulfonic acid)ruthenium(II) (RuBPS)-Ce(IV) system and the effects of two diuretics, hydrochlorothiazide and furosemide, on its chemiluminescence intensity were investigated in detail. It was found that each of the two diuretics could enhance the chemiluminescence emission intensity of RuBPS-Ce(IV) system, based on which, they were sensitively detected by chemiluminescence analysis, respectively. Under the optimum experimental conditions, the linear range and detection limit of hydrochlorothiazide were 2.5 × 10−3 to 6.0 × 10−1 μg ml−1 and 1.0 × 10−3 μg ml−1, respectively; those of furosemide were 1.0 × 10−2 to 4.0 μg ml−1 and 8.8 × 10−3 μg ml−1, respectively. The proposed method has been applied to analyze the pharmaceuticals with satisfied results.