کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743891 | 1491201 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multicomponent determinations using addition-generated reagent profiles and partial least squares regression
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The use of addition-generated reagent profiles is proposed for multicomponent determinations when the reactions between the reagent and analytes are slow. The oxidation of cysteine, methionine and homocysteine with dichromate in acidic medium has been used as the chemical system for experimental verification of some theoretical suppositions. The methodology tries to make the most of the differences in the kinetic behaviour. The continuous addition of dichromate (the reagent) to the sample including the amino acids provides the experimental data when the visible signal from dichromate is followed along time with a diode array spectrophotometer. The whole signal-time profile for the reagent is then used to characterize the sample, and partial least squares regression (PLSR) has been applied as calibration algorithm. Concentrations of amino acids between 10â4 and 10â3Â M have typically been used. In the present case, mean errors increase from individual determinations (between 2 and 5%) to binary (between 4 and 8%) and ternary mixtures (between 6 and 13%). This tendency can be expected to be of general application. Some validation with pharmaceutical products has been accomplished. A critical discussion on the practical interest of this application is finally included.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 535, Issues 1â2, 11 April 2005, Pages 287-295
Journal: Analytica Chimica Acta - Volume 535, Issues 1â2, 11 April 2005, Pages 287-295
نویسندگان
Rosa GarcÃa, Guillermo López-Cueto, Miren Ostra, Carlos Ubide,