کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743920 | 1491202 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel method for determination of peroxynitrite based on hemoglobin catalyzed reaction
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel method for determination of peroxynitrite based on hemoglobin catalyzed reaction A novel method for determination of peroxynitrite based on hemoglobin catalyzed reaction](/preview/png/9743920.png)
چکیده انگلیسی
A novel spectrofluorimetric method for the determination of peroxynitrite is proposed. The method is based on a mimetic enzyme catalyzed reaction with hemoglobin as the catalyst and l-tyrosine as the substrate. A new fluorescent substance is produced that might probably be the coupled dimmer of tyrosine, which, instead of nitryl-tyrosine, is likely to be a new marking substance of ONOOâ injury in vivo. Kinetics of the reaction is studied and the possible reaction mechanism is also recommended. The proposed method is simple and highly sensitive with a detection limit of 5.00 Ã 10â8 mol Lâ1 of peroxynitrite. A liner calibration graph is obtained over the peroxynitrite concentration range 5.60 Ã 10â7 to 2.10 Ã 10â5 mol Lâ1, with a correlation coefficient of 0.9983. Interferences from some amino acids and metal ions normally seen in biological samples, and also some anions structurally similar to ONOOâ are studied.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 530, Issue 2, 14 February 2005, Pages 317-324
Journal: Analytica Chimica Acta - Volume 530, Issue 2, 14 February 2005, Pages 317-324
نویسندگان
Ju Liang, Zhi-Hong Liu, Ru-Xiu Cai,