کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9745085 | 1645297 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Coupling phosphoryl transfer and substrate interactions in protein kinases
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کلمات کلیدی
Npl3SRCpKaCBPSH2SH3CSKSrc homology 2 domain - Src همولوژی 2 دامنهSrc homology 3 domain - Src همولوژی 3 دامنهClamping - بستنRecognition - به رسمیت شناختنKinetics - سینتیک (جنبش شناسی) Phosphorylation - فسفریلاسیونSubstrate - لایهcAMP-dependent protein kinase - پروتئین کیناز وابسته به cAMPKemptide - کمپتییدKinase - کیناز
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Protein kinases control cell signaling events through the ATP-dependent phosphorylation of serine, threonine and tyrosine residues in protein targets. The recognition of these protein substrates by the kinases relies on two principal factors: proper subcellular co-localization and molecular interactions between the kinase and substrate. In this review, we will focus on the kinetic role of the latter in conveying favorable substrate recognition. Using rapid mixing technologies, we demonstrate that the intrinsic thermodynamic affinities of two protein substrates for their respective kinases (Csk with Src and Sky1p with Npl3) are weak compared to their apparent affinities measured in traditional steady-state kinetic assays (i.e.-Km < Kd). The source of the high apparent affinities rests in a very fast and highly favorable phosphoryl transfer step that serves as a clamp for substrate recognition. In this mechanism, both Csk and Sky1p utilize this step to draw the substrate toward product, thereby, converting a high Kd into a low Km. We propose that this one form of substrate recognition employed by protein kinases is advantageous since it simultaneously facilitates high apparent substrate affinity and fast protein turnover.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1754, Issues 1â2, 30 December 2005, Pages 191-199
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1754, Issues 1â2, 30 December 2005, Pages 191-199
نویسندگان
Scot A. Lieser, Brandon E. Aubol, Lilly Wong, Patricia A. Jennings, Joseph A. Adams,