Synthesis and application of mono-6-ammonium-6-deoxy-β-cyclodextrin chloride as chiral selector for capillary electrophoresis

A facile synthetic approach for mono-6-amino-6-deoxy-β-cyclodextrin (β-CD-NH2) was proposed. Its hydroxy chloride salt, mono-6-ammonium-6-deoxy-β-cyclodextrin chloride (β-CD-NH3Cl) was further prepared and used for the enantioseparation of various anionic and ampholytic analytes by capillary electrophoresis (CE). The effect of background electrolyte (BGE) pH and selector concentration on the enantioseparation was studied. Results showed that β-CD-NH3Cl displayed powerful chiral resolution ability towards anionic analytes. In addition, baseline separation of a standard mixture consisting of eight acids was achieved within 35 min.