Enantioseparation of acidic enantiomers in capillary electrophoresis using a novel single-isomer of positively charged β-cyclodextrin: Mono-6A-N- pentylammonium-6A-deoxy-β-cyclodextrin chloride

The new single-isomer of positively charged β-cyclodextrin, mono-6A-N-pentylammonium-6A-deoxy-β-cyclodextrin chloride (PeAM-β-CD), was employed for the first time for the enantioseparation of anionic and ampholytic analytes by capillary electrophoresis (CE). The synthesis and characterization of PeAM-β-CD were reported. The effect of background electrolyte (BGE) pH and selector concentration on the enantioseparation was investigated. Good separation was obtained at low BGE pH (ca. 5.0-6.0). The effective mobilities of all analytes were found to decrease with increasing CD concentration. PeAM-β-CD proved to be an effective chiral selector for most studied anionic analytes.