کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9749219 | 1493804 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of triptolide in root extracts of Tripterygium wilfordii by solid-phase extraction and reverse-phase high-performance liquid chromatography
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of triptolide in root extracts of Tripterygium wilfordii by solid-phase extraction and reverse-phase high-performance liquid chromatography Determination of triptolide in root extracts of Tripterygium wilfordii by solid-phase extraction and reverse-phase high-performance liquid chromatography](/preview/png/9749219.png)
چکیده انگلیسی
Extracts of Tripterygium wilfordii roots have a long history of use in traditional Chinese medicine and have shown great promise in recent clinical trials as a treatment for rheumatoid arthritis. The major active component of Tripterygium root extracts is the diterpenoid triptolide. This paper describes a method for the determination of triptolide in root extracts that is suitable for the analysis of many small samples simultaneously. Extracts are applied to aminopropyl solid-phase extraction (SPE) tubes that are then eluted with dichloromethane-methanol (49:1, v/v). The eluate is chromatographed on a pentafluorophenyl HPLC column using an acetonitrile:water gradient. Triptolide is quantified by ultraviolet detection at 219Â nm. Using this method, it was shown that smaller diameter roots with secondary growth contained higher triptolide concentrations than larger roots. This suggests that roots to be used for production of the drug extract could be harvested while still small, which would reduce the growing time necessary and thus be economically beneficial for the growers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1070, Issues 1â2, 8 April 2005, Pages 65-70
Journal: Journal of Chromatography A - Volume 1070, Issues 1â2, 8 April 2005, Pages 65-70
نویسندگان
Anita M. Brinker, Ilya Raskin,