کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9749332 | 1493807 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-performance liquid chromatographic determination of phenolic compounds in rice
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A method has been developed for the determination of 6â²-O-feruloylsucrose, 6â²-O-sinapoylsucrose, ferulic acid, sinapinic acid, p-coumaric acid, chlorogenic (3-caffeoylquinic) acid, caffeic acid, protocatechuic acid, hydroxybenzoic acid, vanillic acid, and syringic acid in rice. The rice samples were extracted with 70% ethanol, filtered, and defatted. The defatted aqueous solution was subjected to solid-phase extraction using a C18 silica gel cartridge; no analyte was lost in this procedure. The 70% acidic methanol elution was analyzed directly by HPLC and HPLC-ESI-MS. Phenolic compounds were separated with a C18 reversed-phase column by gradient elution using 0.025% trifluoroacetic acid in purified water (A)-acetonitrile (B) (0 min, 5% B; 5 min, 9% B; 15 min, 9% B; 22 min, 11% B; and 38 min, 18% B) as the mobile phase at a flow rate of 0.8 ml/min. Detection limits ranged from 0.10 to 0.35 ng per injection (5 μl). Relative standard deviations of 0.22-3.95% and recoveries of 99-108% were obtained for simultaneous determination of these phenolic compounds. This method was applied to analysis of phenolic compounds in brown rice and germinated brown rice soaked in 32 °C water for varying durations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1063, Issues 1â2, 21 January 2005, Pages 121-128
Journal: Journal of Chromatography A - Volume 1063, Issues 1â2, 21 January 2005, Pages 121-128
نویسندگان
Su Tian, Kozo Nakamura, Tong Cui, Hiroshi Kayahara,