کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9749337 | 1493807 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1:1, v:v). By injecting 200Â mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165Â mg of acteoside and 17.5Â mg of isoacteoside from 978Â mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1063, Issues 1â2, 21 January 2005, Pages 161-169
Journal: Journal of Chromatography A - Volume 1063, Issues 1â2, 21 January 2005, Pages 161-169
نویسندگان
Li Li, Rong Tsao, Zhiqiang Liu, Shuying Liu, Raymond Yang, J. Christopher Young, Honghui Zhu, Zeyuan Deng, Mingyong Xie, Zhihong Fu,