کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9757473 | 1496004 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative determination of serum LDL cholesterol by near-infrared spectroscopy
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Quantitative determination of serum LDL cholesterol by near-infrared spectroscopy Quantitative determination of serum LDL cholesterol by near-infrared spectroscopy](/preview/png/9757473.png)
چکیده انگلیسی
The aim of this study was to assess the feasibility of near infrared (near-IR) spectroscopy for the simultaneous quantitation of the four serum analytes making up the cardiac risk profile, including total cholesterol, triglycerides, HDL cholesterol, and LDL cholesterol. A total of 187 serum samples were obtained randomly from the central clinical chemistry laboratory of a large hospital, and the near-IR spectrum (400-2500Â nm) recorded for each. Triglycerides and total cholesterol were determined using a Hitachi 717 analyzer, with enzymatic, colorimetric detection schemes, and HDL determined via a homogeneous assay on the same analyzer. The LDL cholesterol concentrations were then evaluated for each specimen using the Friedewald formula. Partial least squares (PLS) regression analysis was then used to quantitatively correlate the IR spectra with the reference assays. The near-IR method provides LDL cholesterol levels with RMS difference of 0.43Â mmol/L as compared to the Friedewald approximate values, and total cholesterol and triglyceride levels consistent with previous near-IR studies. No satisfactory calibration could be derived for HDL cholesterol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vibrational Spectroscopy - Volume 38, Issues 1â2, 29 July 2005, Pages 203-208
Journal: Vibrational Spectroscopy - Volume 38, Issues 1â2, 29 July 2005, Pages 203-208
نویسندگان
Kan-Zhi Liu, Minhu Shi, Angela Man, Thomas C. Dembinski, R. Anthony Shaw,